In this study, cobalt ferrite (CoFe2O4) magnetic nanoparticles were biosynthesized using Pedalium murex leaf extract by microwave combustion method (CoFe2O4-MCM) and convention combustion method (CoFe2O4-CCM) for comparative study purpose. Physicochemical properties of the CoFe2O4 nanoparticles were determined by high resolution transmission electron microscopy (HR-TEM), High resolution scanning electron microscopy (HR-SEM), Powder X-ray diffraction (XRD), vibrating sample magnetometer (VSM), and energy dispersive X-ray (EDX) mapping analysis. The XRD diffractograms confirmed the cubic CoFe2O4 nanoparticles. FTIR data showed the presence of the metal-oxygen vibration. HR- SEM images demonstrated the sphere shaped particles with nano range, and EDX further approved the existence of the element. The grown samples were tested for antibacterial disinfection, and CoFe2O4-MCM nanoparticles sample showed a higher zone of inhibition against gram-positive S. aureus (Staphylococcus aureus), B. subtilis (Bacillus subtilis) and gram-negative E. coli (Escherichia coli) and K. pneumonia (Klebsiella pneumoniae) bacteria strains. Furthermore, the results showed that the photocatalytic and antibacterial, performance of CoFe2O4-MCM nanoparticles could be enhanced by particle size and temperature effect. It appears that these nanostructures can be considered as suitable candidates for drug delivery and other biomedical applications, because of their toxicity effects.