Withania Somnifera (WS) is having significant effects on anti-inflammatory, nervine tonic, nerve soothing, antioxidant, immunomodulator, free radical scavenger, adaptogen, antiarthritic, antispasmodic, anti -stress and anti-cancer. But ergogenic value of WS as nutritional supplement is yet to be established. Objectives: Present study was designed to investigate the effect of supplementation of WS on the sprint fatigue level in Hockey Players. Method: Thirty two male hockey players, with a mean age of 17.3 ± 1.8 years and BMI 20.7 ± 2.8 kg/m2 volunteered for the study. Subjects were randomly assigned into two groups Group I (n= 24): Withania somnifera group (experimental group) and Group II (n=24): Placebo ( control) group. The experimental group received 500 mg capsules of aqueous roots of Ashwagandha twice daily for eight weeks, whereas the placebo group received starch capsules. Sprint fatigue level was assessed with Mackenzie B. (2006) 40 Metre Multiple Sprint Test. Test in both experimental and control groups before and after the administration of Withania somnifera and placebo respectively. Results: A significant improvement in the sprint fatigue level after 4 weeks (t = 1.83, p< 0.10, on tail rest) and 8 weeks (t = 2.45, p < 0.02, one tail test) in experimental group was found. Whereas, no significant improvement in the control group for sprint fatigue level after 8 weeks of placebo supplementation was found. Conclusion: Supplementation of Withania Somnifera improves sprint fatigue level in young hockey players.