To develop and validate UV- Spectrophotometric methods for the estimation of Safinamide mesylate in bulk and tablets. Method and materials: In methanol, Safinamide mesylate showed maximum absorption at wavelength 226 nm. In Method A (Zero order spectroscopy) estimation of Safinamide mesylate was performed at 226 nm while in Method B, the zero order derivative spectrum was derivatized into first order derivative spectrum (scaling factor 2) and maximum amplitude at 235 nm was selected for analysis and in Method C, Area under curve of zero order spectrum of Safinamide mesylate was selected in the wavelength range of 227.60-242.20 nm. Results: The drug was found to obey Beer- Lambert’s law in the concentration range of 4 - 24 μg/mL for all three methods. The correlation coefficients were found to be 0.9997, 0.9999 and 0.9997 in Method A, B and C, respectively. Conclusion: All the methods were validated with respect to accuracy, precision and ruggedness. The mean % recoveries were found satisfactory for all three methods. Thus proposed methods have been successfully applied for the estimation of Safinamide mesylate in bulk and tablet dosage form.