The Objective of the study is to assessed the jump shoot accuracy of U-18 male and female basketball players of Indian origin participating at the national level. A total of 60 players (30 males and 30 females) were selected using convenience sampling. The criterion measure for assessing jump shoot accuracy involved each player taking (BJSAT) eight shots from different positions, with scoring criteria ranging from 0 to 3. The collected data was analyzed using descriptive statistics and an independent samples t-test, assuming equal variances. The statistical analysis revealed no discernible variation in the means of jump shoot accuracy between the male and female players. The findings suggest that gender does not have a significant impact on jump shoot accuracy in this specific context. However, it is important to consider the limitations of the study, such as the sample size and possible biases, and Additional investigation is recommended to validate these initial results.