In the current study, the synergistic antimicrobial efficacy of Polygala javana derived extracts in combination with antibiotics has been assessed on selected pathogens using different solvent extracts. Stem, root, flower and leaf derived powders of Polygala javana were extracted by using Soxhlet apparatus using the solvents viz., acetone, benzene, ethanol n- butyl alcohol and isopropyl alcohol against five bacterial strains such as Gram positive bacteria Staphylococcus aureus and Gram negative bacteria Escherichia coli, Klebsiella pneumonia, Salmonella typhi, The standard drug, Ciprofloxacin 10ug/disc was used as control and zone of inhibition ranged between 23mm and 43mm. The N-Butyl Alcohol extract recorded the highestzone of inhibition in all extract of Escherichia coli at 1000ug/disc. acetone extract against Proteus vulgaris at 250µg/disc concentration showed lowest mean of zone of inhibition. Synthetic discs with the antibiotics Chloramphenicol, Tetramycin, Ampicillin, and Gentamycin respectively were used for comparison All the tested human pathogens were highly sensitive to Gentamycin with the zone of inhibition above 20mm. The antibacterial activity of commercial antibiotics was reported to be higher than that of the plant extracts.