Strychnos nux-vomica L. commonly known as ‘Nux-Vomica, Poison nut and Strychnine tree in English is a medium sized deciduous tree which belongs to the family Loganiaceae. Strychnos nux-vomica L. traditionally has been used for treatment of various ailment since generations. These seeds have been found to be rich in protein and possess high calorific value. Proximate and Ultimate analysis of seed reveals Moisture – 5.21%, Ash – 11.72%, Volatile Matter – 80.22%, Fixed carbon – 2.85%, N – 0.82%, C – 51.05%, H – 9.433%, S – 0.10%. The Strychnos nux-vomica L. seeds are enriched in mineral and nutrient content which are rich source of K and Ca ions. Quantitatively, the amino acid profile by HPLC result shows essential amino acids such as methionine, threonine, leucine, isoleucine, lysine, phenylalanine, histidine (EAA - 29.1 %) and non-essential amino acids such as glutamate followed by serine, glycine, tyrosine, aspartate, proline, cystine, arginine (NEAA - 62.68%).