Nostoc muscorum is an oxygenic photosynthetic, obligate photo autotrophic, gram negative, free living green-brown coloured cyanobacteria found in both terrestrial and aquatic environmental condition. It is rich in secondary metabolites but least investigated. The present investigation was aimed at extraction of bioactive chemical mixture from N.muscorumgrown in media with and without ammonium chloride. The species was used to culture in Chu no.10 medium then itsdried fine powder was maceratedmethanol for extraction