Nanotechnology brought everlasting wonders in the field of health science and medicine. It extends its role in other fields such as textile industries, engineering and electronics, defense and security, cosmetics and energy storage. This vast field is steered up by the involvement of nanoparticles. Metal nanoparticles are distinctive and they show a great range of advantages in diverse disciplines. Nanoparticles are obtained via different approaches like physical, chemical and biological methods. Synthesis of metal nanoparticles by biological method is an ecofriendly access to move in to the extraordinary uses of the same. Noble metal nanoparticles are highly beneficial especially in the drug delivery and therapeutic techniques. Gold nanoparticles, especially have indigenous medicinal features in curing different diseases. One pot green synthesis of nanoparticles are common nowadays and it turns down the side effects created by other usual treatments. Various Biosystems are being used for the synthesis of nanoparticles including prokaryotes and eukaryotes. Edible mushrooms are the richest source of nutritional constituents principally antioxidants, fibers and proteins. Laetiporus versisporus is one such mushroom which was used for the synthesis of gold nanoparticles in the current study. Earlier studies reported the isolation of Lanostane Triterpenoids and Saponins from Laetiporus versisporus. Synthesized gold nanoparticles were characterized by UV-Vis Spectroscopy, X-ray diffraction (XRD), Fourier Transform –Infrared spectroscopy (FT-IR), SEM and TEM. They were then investigated for their anticancer activity against breast cancer cells (MCF-7) and found to be having greater cell inhibition percentage.