The present study aims to evaluatethe antioxidant potential and antibacterial activity of crude extracts from root and leaf parts of Catharanthus roseus against periodontalpathogens of clinical importance.Crude extract from root and leaf part of C.roseus involved active principles in appropriate solvent followed by evaluation of antibacterial activity by agar well diffusion assay against selective bacterial species. To determine minimum inhibitory concentrationfor the crude extracts of C.roseus.Among the extracts that were significantly active, extract obtained using chloroform and methanol exhibited maximum activity against bacterial species tested.Gram (-) bacteria were more sensitive when compared to Gram (+) bacteria. The study also focuses the determination of antioxidant activity and flavonoids, alkaloids terpenoids, and total phenolic content (TPC) of C. roseuscrude extractusing DPPH assay and IC50 (Inhibitory concentration) using broth dilution assay. Phytochemical screening revealed that leaf and root extracts of C. roseus is a potential source for anti-leukemic alkaloids and phenolics. This study implicates that leaf and root extracts of C. roseus could potentially be exploited as therapeutic agents for various bacterial infections.