Objective: The present study was aimed on developing and characterizing Liposomal Delivery System loaded with antigen of filaria parasite B. malayi extracted protein for assessment of Cell Mediate Immune responses of antigen. Methods: Liposomes were prepared by Reverse Phase Evaporation (REV) method (Szoka and Papahadjopolus; 1978) with slight modification using molar ratio of Soya PC:PE:Cholesterol in different molar concentration. Results: NO release from peritoneal macrophages of the animals (Gr.I, II, III, IV and V) was increased by exposure to LPS or no exposure to any stimulants in-vitro as compared to cells of non-immunized animals (Gr.V). In summary, F6 was able to induce greater NO production. The TNF-α release in cells of F6 immunized animals was elevated in response to F6, LPS or no stimulation in-vitro over non-immunized ones. The IFN-γ release in cells of F6 immunized animals was elevated in response to F6 or without any stimulation in-vitro in comparison to non-immunized ones. Up-regulation in Th-I responses and down-regulation in Th-II responses show that the immunological cytokines were in function and cause triggers to body immunity to destroy the parasite, the cytokines production checked at mRNA transcription level using RT-PCR.