Large amount of chemical fertilizers are commonly used in agricultural sector for enhancing the crop productivity to meet the global food demand which also plays a major role to deteriorate environment as well as human health. The use of microbial bioformulations for crop cultivation particularly raw vegetables is a good practice considering human health and environmental sustainability. The present study was carried out to analyze the effect of microbial bioformulations on the plant growth, biochemical profile (chlorophyll, carotenoids and protein content) and defence related compounds (flavonoids and phenolic content) of spinach. In this study, a bioformulation consisting consortium of four bacterial strains (Bacillus filamentosus RS3B, Bacillus pseudomycoides RS6B, Bacillus paramycoides RPB3, Alcaligenes faecalis RS10B) in two carrier material viz. Bentonite and Talc+Gluten named as T1 (Bentonite+microbial consortium (MC)) and T2 (talc+gluten +MC) along with inoculated broth medium(T3) uninoculated carriers (T4 and T5), chemical fertilizer (T6) and non-inoculated soil (T7) were applied for the cultivation of spinach crop. The results depicted that inoculation of bioformulation containing MC considerably increased the concentration of total phenolics in both bentonite based (T1) and talc+gluten based bioformulation(T2) by 48.7% and 100.43%, the flavonoids were also increased by 235.02 and 310.46%, total chlorophyll 71.8-162%, carotenoids 156-209.5%, protein 35.2-87% and dry biomass by 275-703% over untreated control. Therefore, our findings indicate, that carrier-based bioformulations (T1 and T2) are excellent plant growth regulators, which increase the yield and overall quality of spinach, an iron rich common vegetable.