Nano-technology has newlyoccurred as a fastrising field with many bio-medical science applications. By the time, silver has been accepted as an anti-microbial material showingfree radical scavenging activity that is relatively free of adverse effects. Therefore, this research has been carried out to estimate the anti-microbial and anti-oxidant activity of Crataevanurvalaleaf extract and silver nanoparticle (AgNPs) synthesized by Crataevanurvalaleaf (CNL-AgNPs). The characterization of AgNPs was done by using by using Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). The anti-microbial activity of CNL-AgNPsand methanol extract of C. nurvalaleaf (CNLM) were tested against bacterial strains such as P. aeruginosa, S. aureus, E. coli,andB. subtilisand fungal strains like C. albicans, T. reesei, A. niger, and P. chrysogenum using the well diffusion technique. The prominent transmission band in FTIR spectra was observed at 3435 cm-1 (>N-H-), 1689 cm-1 (Quinone or conjugated ketone) and 1083 cm-1 (Si-O-Si). The range of particle size in SEM was between 29-82 nmwith spherical shape. CNL-AgNPs showed more diameter of inhibition against Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus subtiliswith an inhibition zone of 16mm, 12mm, and 15 mm respectively when compared with CNLM. CNL-AgNPsexhibited prominent antifungal properties against Candida albicans, Aspergillus nigerwith 16mm inhibition diameter. Additionally the highest catalase (0.062 µM H2O2 reduce/gm Fwt/sec), peroxidase (0.558 µM/L/gm dwt/sec) and FRAP (1.799 µM at 100 µl) antioxidant activity were also observed with synthesized AgNPs in comparison to CNLM. Overall, the results concluded that the synthesized silver nanoparticle of C. nurvala leaf was more effective as antimicrobial and antioxidant agents as comparedto C. nurvalaleaf.