The main aim of this study is to evaluate the antioxidant, anti-inflammatory properties and FT-IR analysis of Hydrocotyle verticillata methanol extract. The antioxidant activity like DPPH free radical scavenging activity, Hydroxyl radical scavenging assay, superoxide anion radical scavenging assay and nitric oxide radical scavenging assay was undergone for the extracts of H. verticillata. The methanol extract was studied for in vitro anti-inflammatory activity in the morphological changes of RAW 264.7 cells were visualized. Anti-inflammatory activity Inhibition of cyclooxygenase enzyme activity of maximum inhibition of (36.36%), lipoxygenase activity (33.46%), Myeloperoxidase (0.001353 U/ml), Inducible Nitric Oxide Synthase (48.50%) and Estimation of cellular nitrite levels (932.085 µg). FTIR analysis was undergone and the major peak attained for H. verticillata methanol extract in peak 2924.40 cm-1 functional groups Alkanes (-CH2-).