IJFANS International Journal of Food and Nutritional Sciences

ISSN PRINT 2319 1775 Online 2320-7876

Antimicrobial Screening Of Carboxymethylated Caesalpinia Pulcherrima Galactomannan

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Nilima Thombre, Shubham Khairnar, Vivek Jadhav, Sandhya Dhavale, Abhishek Jadhav, Rutuja Jogdand, Suman Choudhary

Abstract

Caesalpinia pulcherrima galactomannan (CPG) is a natural polysaccharide consisting of a (1→4)-linked α-D-mannopyranosyl backbone, substituted at O-6 by single units of β-D-galactopyranose. Literature reveals that the carboxymethyl group is the key factor responsible for increasing antibacterial activity. Current study focussed on screeniong of in vitro antimicrobial properties of carboxymethylated galactomannan (CMCPG) against gram-positive and gram-negative bacteria (S. aureus and E. coli). Derivatization of galactomannan was performed by using a carboxymethylation reaction with variation of reaction conditions such as the concentration of monochloroacetic acid and temperature. Optimized derivative were evaluated for degree of substitution(DS), FTIR, percentage yield and solubility. Minimum Inhibitory Concentration (MIC) was tested by using the liquid dilution method whereas zone of inhibition (ZOI) was evaluated by the well diffusion method. Synthesised carboxymethylated derivative detected with degree of substitution of 0.56 ± 0.05, percentage yield of 144.02 ± 1.5 % and solubility of 80.96 ± 1.3 %. FTIR spectrum of CMCPG detected with presence of the characteristic peak at 1589 cm−1 and 1319 cm−1 which confirmed introduction of carboxymethyl group into the molecular structure of CPG. Absentism of in vitro antibacterial activity detected in range of concentration 50 mg/mL to 0.78 mg/mL for CMCPG alongwith lacuna of MIC in range of concentration 13.75 mg/mL to 1.25 mg/mL). As per the screening of Carboxymethylated Caesalpinia pulcherrima Galactomannan, zone of inhibition, minimum inhibitory concentration and antibacterial activity against gram negavtive E. coli and gram-positive S. aureus. Thus, screened carboxymethylated Caesalpinia pulcherrima derivative may not be a promising antimicrobial agent.

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