The aim of this study is to evaluate the anti-diabetic activity of lycopene extracted from tomato manufacturing waste streptozotocin -induced diabetes in male rats. Thirty five (35) male albino rats (twenty eight diabetic and seven normal control rats) were used. The rats were divided randomized into five groups of seven rats each: G I: Normal control rats that fed only normal basal diet and water. G 2: diabetic control rats that fed only normal basal diet and water. G 3: Diabetic rats were fed on basal diet and treated with 10 mg lycopene /kg body weight. G 4: Diabetic rats were fed on basal diet and treated with 20 mg lycopene /kg body weight. G 5: Diabetic rats were fed on basal diet and treated with 10 mg lycopene /kg body weight. (G3), (G4) and (G5) were treated with lycopene orally once per day for 4 weeks. Diabetes was induced by a single intraperitoneal injection of streptozotocin 60 mg/kg body weight and it was confirmed by the elevated blood glucose ≥ 200 mg/dl after three days. Based on the obtained results, the diabetic control rats (G2) showed high blood glucose level, cholesterol, triglyceride, low-density lipoprotein (LDL-C),very low-density lipoprotein(VLDL-c) , malondialdehyde (MDA) , as well as urea, creatinine and uric acid. On the other hand, the levels of insulin production, high- density lipoprotein cholesterol (HDL-c) and antioxidant enzymes were significantly decreased in the diabetic control rats (G2) compared with the normal control rats (G1). treatment of graded doses of lycopene to diabetic rats had significantly (P<0.05) reduced blood glucose level, cholesterol, triglyceride, low-density lipoprotein (LDL-c), very low-density lipoprotein(VLDL-c), malondialdehyde (MDA), as well as urea, creatinine and uric acid. In addition, the levels of insulin production, high- density lipoprotein cholesterol (HDL-c) and antioxidant enzymes, such as catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and Glutathione-L-reduced (GSH) were significantly increased in the treated diabetic rats with lycopene extracted from tomato manufacturing waste at dose 20 mg/kg (G4) and 30 mg/kg (G5) respectively compared with the diabetic rats (G2).