Two endophytic fungi (ASUF11and ASUF23) were confined from the plump leafs of seaweed Ulva faciata collected from south peninsular bank of India and were removed by utilizing standard methodology. The antibacterial exercises of concentrates were completed against normal bacterial microbes. Both the concentrates displayed huge bactericidal action in all microorganisms. The methanolic concentrates of ASUF23 showed wide range antibacterial movement (70%) in both gram positive and gram-negative microorganisms than different gatherings.