Nostoc muscorum is a free-living microbe that can be found all over the world in a wide variety of terrestrial and aquatic settings. It is popular in desert crusts and benthic communities of water-logged paddy fields and is known to create symbiotic partnerships with terrestrial plants. N. muscorum prefers environments with pH values between 7.0 and 8.5. It was cultured in a medium with a pH range of 7 to 11, and its morphological and physiological traits, such as the frequency of heterocysts and the amount of the pigment phycobilin, were assessed. The findings show that N. muscorum can develop and carry out its physiological functions at an alkaline pH range of 8 to 10.