Jhunjhunu district, located in the Indian state of Rajasthan, possesses a rich diversity of flora, including numerous ethno-medicinal plants. These plants have been traditionally used by the local communities for various medicinal purposes, contributing significantly to the indigenous healthcare system. This paper aims to document the ethno-medicinal plants of Jhunjhunu district, highlighting their traditional uses, medicinal properties, and conservation status. Through field surveys, interviews with local healers, and review of ethnobotanical literature, a comprehensive list of ethno-medicinal plants has been compiled. Additionally, the paper discusses the importance of conserving these plant species for sustainable healthcare practices and biodiversity conservation in the region.