India's biodiversity is full of nature's perspective. In respect to the plant species of this biodiversity, it is very important to do scientific study of wild plants in which useful phytoalexin compounds are present in abundance. Hence, in the present study, we mainly aimed to establish rationale for ethno pharmacological uses of Blumea lacera (Burm.f.) DC accessions collected from difference geographical locations in India. The quantification of precocene and caryophyllene oxide contents in water and ethanolic extract of different accessions of Blumea lacera (Burm.f.) DC collected from different geographical locations in India was caried out using HPLC methods. The results of quantification of Precocene I and II in different accessions of Blumea lacera delineated that the Blumea lacera accession CVP-05-04 contains highest quantity of Precocene I i.e., 0.053% followed by CVP-05-12 (0.047%) and CVP-05-03 (0.044%). The results of quantification of Caryophyllene oxide in different accessions of Blumea lacera revealed that Blumea lacera accession CVP-05-04 contains highest quantity of Caryophyllene oxide i.e., 0.053% followed by CVP-05-12 (0.047%) and CVP-05-03 (0.044%). In conclusion, presence in highest quantities of Precocene I and Caryophyllene oxide in CVP-05-04 accession of Blumea lacera would be responsible ethnopharmacological activities of Blumea lacera accession CVP-05-04. Hence, CVP-05-04 variety of Blumea lacera could be explored for ethnopharmacological uses.