Humans established relations with food for their existence on earth. In various parts of the world, different types of vegetation are used as food material. To overcome the crises in food, the alternative source of food may be the direct product of plants which can be consumed in raw condition. Considering the growing need to identify the alternative sources, most of the people are consuming leafy vegetables as major food source. To see the nutritional importance of leafy vegetables, most are cultivating now a days for both as food and their livelihood. A small work was conceptualized and undergone by the BSc final year students of the Biotechnology department, NIIS Institute of Information Science and Management, Bhubaneswar. The present study evaluated the moisture content, total protein and total carbohydrate content in common leafy vegetables used in Odisha. Total protein was estimated by Lowry method, while total carbohydrate and free amino acid content by anthrone method and spectrophotometric method. The result showed highest protein contained in Spinacia oleracea (103.98 ± 2.34 µg/mg) followed by Moringa olerifera (99.86 ± 2.32 µg/mg), Centella asiatica (95.53 ± 2.78 µg/mg). The highest carbohydrate content was observed in the leaf of Amaranthus viridis (116.26 ± 2.33 µg/mg of plant tissue) followed by Ipomoea aquatica with carbohydrate content (82.35 ± 1.55 µg/mg), Basella alba with carbohydrate content (50.46 ± 2.23 µg/mg), and highest moisture content was recorded in the leaves of (89.57 ± 18.44%) followed by Spinacia oleracea (89.32 ± 19.12%), and lowest in Bauhinia purpurea (30.45 ± 15.55%). The study’s finding confirmed that wild leafy vegetables are having potential nutritional value and these can be cultivated for commercialization purpose. The cultivation of these leafy vegetable plays a significant role in their livelihood and sustainability. As per the data reported, more than one million people of this region suffer from malnutrition so present study is an approach to overcome the health issue of people of this region in a sustainable manner.