Herbal medicines are becoming increasingly popular. Herbal medicines for skin disorders are gaining popularity due to the belief that they are safer and have fewer side effects than other treatments. The primary goal of this study was to create and test antimicrobial polyherbal ointments containing leaf extracts of Neem (Azadirachta indica) and Tulsi (Ocimum tenuiflorum). Cold maceration was used to create the hydroalcoholic extracts. The various ointment bases were prepared. They were evaluated for physicochemical parameters such as nature, colour, odour, pH, spreadability, extrudability, consistency, skin irritation, and gave satisfactory results after formulation. For antimicrobial activity against Staphylococcus aureus, the best formulation was used. The prepared formulation's antimicrobial activity was compared to that of a commercial formulation (10% w/w betadine). The overall result of this study reveals that this is an effective polyherbal antisptic ointment.