A pot experiment was conducted to investigate the tolerance of nickel by three Indian mustard plant genotypes, namely, RH - 819, Varuna and Rh-9304, in a light soil from sand dune areas of Balsamand, Hisar. Five levels of Ni concentration ranging from 0-120 mg kg-1 soil were taken for the study. The toxicity symptoms were recorded; biomass production, Ni concentration and finally the Ni uptake were measured to screen the best Ni tolerant Indian mustard genotype. The plants were harvested at maturity. The results of this study showed that all the three Indian mustard genotypes survived upto Ni 200 mg kg-1 soil level. The genotypes exhibited visual Ni toxicity symptoms with narrow and small leaves, stunted growth, poor branching, delayed flowering and poor seed development and resulted into low biomass yield. Genotype RH-819 produced highest mean biomass, Ni concentration and its uptake on Ni spiked soil. Hence, genotype RH-819 of Indian mustard was found the best Cd and Ni tolerant genotype and was selected for uptake study.