Carcinoma of the prostate is the most common malignant tumour in men over the age of 65 years with peak age of incidence between 70 and 74 years. Oxidative stress and one carbon metabolism are important contributory factors in etiopathogenesis in Prostate Neoplasm. Significant increase in oxidative stress and serum homocysteine level and no difference of serum vitamin B12 was found in BPH and prostate cancer cases compared to control group. Following study was conducted to compare level of Oxidative Stress Marker and One Carbon Metabolism markers between Prostate neoplasm patients and control Group. We concluded that Homocystine and Vitamin B12 were high in patients with prostate cancer as well as BPH patients when compared with age matched healthy controls.