Background- Corchorus olitorius and Corchorus trilocularis are the medicinal plants belonging to family Tiliaceae found in western Rajasthan. Aims-The Physico-chemical characteristics, fatty acids composition, antioxidant potential and total phenolic content of the two unexplored Corchorus species (C.olitorius and C.trilocularis) fatty acids methyl esters (FAMEs) seed oils from Rajasthan were analyzed. Methods and materials-The seed oils of both the Corchorus species were extracted by using soxhlet apparatus. The fatty acid compositions were determined by using GC-MS technique. The antioxidant potential were analyzed by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and Ferric reducing ability of plasma (FRAP) methods. Results and conclusion-The C.olitorius seed oil was reported with higher amount of oleic acid ( ) as compared to C.trilocularis ( ). The results confirmed that both the Corchorus species have significant antioxidant properties. C.olitorius have the higher trolox equivalent antioxidant capacity ( ) than in C. trilocularis (). Similarly DPPH activity reported in C.olitorius FAMEs seed oil is quite higher than in C.trilocularis. The total phenolic content in C.olitorius and C.trilocularis were and respectively. These results suggested that both these Corchorus species could be employed as a possible source of monounsaturated fatty acids and also phenolic components with antioxidant properties in the food industry, and pharma sectors.