In this present work, BiFeO3 (BFO) and La0.05Bi0.95FeO3 (LBFO) nanoparticles have been synthesized using urea as a fuel via sol-gel combustion method. The synthesized materials have been analyzed by Powder X-ray diffraction (XRD), Fourier- Transform Infrared spectroscopy (FT-IR), Ultraviolet-visible (UV-Vis.) spectroscopy, High-resolution scanning electron microscopy (HR-SEM), HR-TEM, Magnetic measurements and antimicrobial studies. The X-ray diffraction patterns (XRD) of BFO demonstrates the rhombohedrally distorted perovskite structure with the space group R3c. Furthermore, the average particle size of BFO and LBFO were found to be 19 and 17nm respectively. The morphological studies of BFO and LBFO revealed the formation of sphere shaped nanostructures with uniform grain size. Also, it was found that the grain size was decreased by doping La contents to BFO. The magnetic measurements showed a ferromagnetic behavior of LBFO and the saturation magnetization drops drastically from 7 emu/g for BiFeO3 up to 6 emu/g.