Indigofera linifolia (L.f.) Retz (Fabaceae) is an erect, prostrate herb that spread throughout India and was found to be used by people to cure febrile eruptions. The extracts of leaves showed antibacterial and antifungal activities. The current study assessed the phytochemical analysis of the three solvents resembling petroleum ether, ethanol, and aqueous extracts, which were examined for eight phytochemicals. Petroleum Ether contains five phytochemicals among the 8; Ethanol contains five phytochemicals, whereas aqueous includes four. The Antibacterial activities of ethanol extract of Indigofera linifolia (L.f.) Retz. Showed a higher zone of inhibition against the selected bacteria Staphylococcus aureus (21mm), E.coli (15mm), Pseudomonas aeruginosa (14 mm), Bacillus subtilis (11mm), and the control microbial antibiotic Amikacin is the less inhibition zone (20mm). Meanwhile, the antifungal activity of ethanol extract from Indigofera linifolia (L.f.) Retz. Showed a higher zone of inhibition against the selected fungi Candida tropicalis (17mm), Aspergillus niger (16mm), and Candida albicans (14mm). The results of the current study will create a way to invent herbal medicines for several ailments by using Indigofera linifolia (L.f.) Retz, which may lead to the development of novel drugs. The presence of secondary metabolites has been identified from the phytochemical studies. Hence, this plant may be used to treat many diseases.