Recent metagenomic investigations of settings such as marine and soil have greatly improved our knowledge of the various microbial communities that live in these habitats including their vital roles in the maintenance of large ecosystems. The rise in publications related to soil and marine metagenomics contrasts sharply with those pertaining to airborne microbes, despite the fact that airborne bacteria are thought to have a substantial impact on many aspects of life, from their potential roles in atmospheric events including such cloud formation, precipitation, and atmospheric chemistry to their major impact on human health. We will address recent advances in airborne metagenomics in this review, with a particular emphasis on the difficulties and possibilities of doing such investigations. The following are the major obstacles to performing metagenomic investigations of airborne microbes: Low microbe density in the air, effective recovery of microorganisms from the air, heterogeneity in airborne microbial community, lack of defined procedures and methodologies, including DNA sequencing or bioinformatics-related difficulties are all factors to consider. Novel genes and metabolic pathways important to meteorological as well as industrial applications, environmental bioremediation, including biogeochemical cycles may be discovered as a result of airborne metagenomic research.