The aim of this study was to determine probable Helicobacter Pylori infection in recurrent apthous stomatitis samples by Rapid Urease Test (RUT). Materials and Methods: This in vivo cross-sectional study was approved by the Ethics Committee of Rama Dental College Hospital and Research Centre according to the ethical standards. A total of 30 patients with minor recurrent aphthous stomatitis and 20 healthy control groups were included in the study. Results: Out of 30 patients with minor recurrent aphthous stomatitis, including 14 male and 16 female patients, with mean age of 47 and 38 years respectively, 19 patients (68%) were RUT (positive). Out of 20 healthy control groups, 10 males and 10 females, 3(11%) were RUT (positive) [Table 1]. Conclusion: HP may play a role in the etiology of RAS; it is likely that RUT may be rapid and reliable for investigation of HP in RAS lesions. Conclusion: compared to healthy individual there was higher prevalence of H.pylori found in patient with recurrent Apthous stomatitis