In vivo and microchemical analytical methods play crucial roles in advancing our understanding of plant metabolism and development. These methods allow researchers to study biochemical processes and molecular interactions within living organisms at a cellular or subcellular level, providing valuable insights into plant physiology. Benchtop microprobe X-ray fluorescence spectroscopy (m-XRF) is indeed a promising tool in this regard. m-XRF provides high spatial resolution, allowing researchers to analyze elements within specific regions of plant tissues. This capability is essential for understanding the distribution and accumulation of elements involved in plant metabolism. Unlike some traditional analytical techniques, m-XRF is non-destructive, meaning it can analyze samples without altering their integrity. This feature is particularly valuable when studying living organisms like plants, where preserving sample viability is essential for accurate results.They can detect and quantify a wide range of elements present in plant tissues. This capability is useful for studying nutrient uptake, mineral accumulation, and elemental composition changes during various stages of plant development.By mapping the distribution of multiple elements simultaneously, m-XRF enables researchers to correlate elemental patterns with physiological processes. This holistic approach provides comprehensive insights into the dynamics of plant metabolism and development.Benchtop m-XRF instruments offer relatively fast analysis times compared to some other techniques. This efficiency is advantageous for high-throughput studies or when analyzing multiple samples, allowing researchers to generate data more rapidly.