Objectives: Many diseases are associated with the accumulation of free radicals and antioxidants can scavenge free radicals and minimize their impact. Therefore, the search for naturally occurring antioxidants of plant origin is vital. This study aimed to investigate the antioxidants and free radicals scavenging properties of ethanolic extracts from Tribulus terrestris leaf (TTL). Materials & Methods: The antioxidant and free radical scavenging activities were measured by using standard protocols against phytoextract used at different concentrations. Total phenolic and flavonoid contents were estimated using Folin- Ciocalteu and aluminum- chloride reagent assay methods, respectively. Results: TTL extract showed the highest total antioxidant ability and with regard to various scavenging activities, and showed the highest radical scavenging activity closely resembled the standards. The quantified phenolic and flavonoid contents of TTL ethanolic extract was19.0±0.16mg of gallic acid equivalent per gram extract and 26.6±0.15mg quercetin equivalent per gram extract respectively. A positive correlation (p< 0.001) was observed between phenolic content and free radical scavenging efficiencies. Discussion: The results confirm that TTL is important sources of natural antioxidants and serves as effective free radical scavengers and/or inhibitors. Hence, TTL extract is suitable plant-based pharmaceutical product can be used to handle free radical-mediated diseases.