The objective of this study is to evaluate the physiological and physical fitness characteristics of female hockey players competing at the National and State levels. A purposive sampling method selected a total (N= 40) of female hockey players from Punjab State. These players are then divided into two groups: National level Players (n1=20) and State level Players (n2=20). The study focuses on measuring physiological variables such as breath-holding capacity, and resting pulse rate, as well as physical fitness variables including speed, and agility. Statistical techniques such as Mean, Standard Error of Mean, and Mean difference are used to analyze the collected data Variables. According to the study’s results, national-level female hockey players ranked higher than their state-level female hockey in all physical activity categories and all physical fitness categories.