Breath plays central role at the psychophysiological level due to its relationship between mind and body. The essential role of breath is employed in the practice of Anapanasati meditation technique. Current study aims to observe the nostril dominance in male and female meditators as reflected in electrophotonic imaging parameters. Methods: A total 432 subjects (264 male and 168 females - mean age 34.36±6.83) were part the current study of two meditation places. Long Term Meditators (LTM) had more experience of meditation than 60 months (mean of months 111±47.20,hours per day 1.71±1.20). Short Term Meditators (STM) had experienced meditation from six months to less than 60 months (mean of months 37.17±19.44,hours per day 2.14 ±4.99 ). Cross-sectional design was used in the study. Data were collected from two sites using Electrophotonic Imaging (EPI). Result: Overall results in this study show that long-term and short-term male and female practitioners had right hemispheric activation as compared to left during the time of assessment. There was no uniform trend of nostril dominance in results towards either direction in both LTM and STM groups. Observation of nostril was contributory in understanding trends in subtle energy changes in meditators. Conclusion: Long-term male meditators showed right hemispheric dominance at the psychophysiological level at the time of assessment. Physiologically and psychophysiologically long and short term female meditators exhibited fluctuating trends in both directions-right and left hemispheric dominance as reflected in the electrophotonic imaging parameters