Volume 13 | Issue 4
Volume 13 | Issue 4
Volume 13 | Issue 4
Volume 13 | Issue 4
Volume 13 | Issue 4
Using high performance liquid chromatography (HPLC) and ultraviolet absorbance detection (UV), a straightforward method for measuring carbamazepine was created. Liquid-liquid extraction and protein precipitation are the two phases in the procedure. The internal standard (is) in this study was diclofenac sodium. An analytical Thermo C8 (250 x 4.6 mm), 5 µm column was used for the separation, and the mobile phase was made up of acetonitrile, isopropyl alcohol, and phosphate buffer pH 3 (36:15:49). There was a 1.2 mL/min flow rate. At 220 nm, the eluent was observed, with a sensitivity level of 0.05 absorbance units. It was possible to produce a linear detection response for values between 0.1 and 8.0 µg mL−1. 0.1 µg mL−1 was the limit of quantitation (LOQ). The suggested HPLC approach is easy to use, quick to perform, extremely sensitive, and has been validated for the accurate detection of carbamazepine. It may also be a dependable method for human pharmacokinetic research.