A study on Mathematical Models an Artificial Neural Networks (ANN) for Prediction of Cellulose hydrolysis is a complicated process due to the numerous inhibitory and enzyme inactivation processes that take place during the course of hydrolysis. When it involves building predictive fashions for tactics containing complex response kinetics that might in any other case be not possible to simulate the usage of extra widespread deterministic approaches, Artificial Neural Networks (ANNs) are extraordinarily effective. The present day research turned into done so as to research the software of ANN as a device for predicting glucose manufacturing with the aid of using enzymatic hydrolysis of natural cellulose, in addition to the contrast of the anticipated glucose manufacturing with mathematical fashions and experimental findings. In order to forecast glucose manufacturing, a feed ahead neural community with one hidden layer turned into educated and utilised to make predictions.