A hectic schedule and demanding tasks challenge nurses to adjust their lifestyle and dietary habits. The objective of this study is to assess the lifestyle pattern, dietary habits they followed and to ascertain the nutritional status of nurses. Methods: This is a cross sectional, comparative study, using purposive sampling technique. The sample consisted of registered female nurses (N=500) aged 25-45years working in government and private hospitals selected from rural and urban areas of Thiruvananthapuram city. The lifestyle pattern of the nurses was elicited through a schedule by eliciting the details on physical activities, sleep patterns, quality of social and family relationships. Anthropometry, biochemical, dietary and clinical assessment methods were used to elicit their nutritional status. Results: Nurses in both sectors were following unhealthy lifestyles. They had no physical exercise, no sufficient sleep, no adequate work breaks, and most of them found it difficult to keep a balance with work and home due to their busy schedules. There was prevalence of underweight and overweight among nurses along with their poor dietary habits. Biochemical analysis of blood samples indicated the prevalence of anaemia, diabetes, and hypercholesterolemia. Conclusion: This present study has identified there is a large lacuna in lifestyle and health profile of nurses. There is an urgent need by hospital managements and policy makers to ensure quality nursing service through staff development and training programs.