Background: Immunization is one of the most cost-effective public health interventions that reduce morbidity and mortality from vaccine preventable diseases. Commitment from health care workers and care givers of children is absolutely essential to achieve100% immunization in India. Objective: To assess the knowledge, attitude and practices of the mothers about vaccination of new-borns in a rural community of Ghaziabad. Methodology: This community-based cross-sectional study was conducted on mothers in a rural community of Ghaziabad to assess the Knowledge, Attitudes and Practices about vaccination in Santosh Medical College, Ghaziabad. For data collection a semi-structured study schedule for in-depth interview of the mother of the children was used as the tool. The respondents were interviewed to record the various socioeconomic variables and the knowledge and practice of the mothers of the children regarding vaccination of new-born. Result: All findings of this study pertain to the 300 mothers surveyed in the selected villages of Muradnagar block, Ghaziabad district. Most 251 (83.7%) mothers had knowledge that vaccines are necessary to protect the new-born from infection or disease. 267 (89%) children received BCG, 263 (87.7%) received OPV0 and 189 (63%) children received Hepatitis B-first dose at birth. Conclusion: The study concluded most of the mothers had knowledge that vaccines are necessary to protect the new-born from infection or disease but more than half of the mothers were unaware of the national immunisation programme. At birth BCG, OPV zero dose and Hepatitis B should be given as per Universal Immunisation Programme.