In the present study protease enzyme producing bacteria was isolated from blood swab samples collected from butcher shop material. The isolates were identified by morphological and biochemical characteristization. The bacterial isolates identified were E.coli and Salmonella sp. These isolates were screened for the production of protease enzyme and then transferred to the production media for enzyme protease production. These enzymes can be extracted further by downstream processing and applied in the industries such as leather, detergent, pharmaceuticals and textiles. The enzymes that are of microbial origin are really cost effective than artificial enzymes that are man-made.