The inflammation in the rectum and anal regions is largely attributed to the nuclear factor kappa beta (NF-κB) signaling pathway, which stimulates the expression of matrix metalloproteinase (MMP9) and inflammatory cytokines like tumor necrosis factor (TNF-α) and interleukin-1β (IL-1β). While previous research has focused on NF-κB and MMP9 pathways, there is a need to explore target mediators in the context of inflammatory ulcer healing and NF-κB pathway. To understand the binding properties of TMF and EGCG with the target proteins, docking studies were performed using PyRx and other available software. Subsequently, the synergistic ulcer healing and anti-arthritic effects of MFS were investigated using DSS-induced colon ulcers in Swiss albino rats. The analysis involved assessing colon mucosal injury through colon ulcer index (CUI) and anorectic tissue microscopy. ELISA and Western blotting were used to determine the expression of IL-1β, TNF-α, pERK, MMP9, and NF-κB in the colon tissue. Additionally, RT-PCR was employed to measure the mRNA expression of inflammatory marker enzymes.