Metallic nanoparticles are widely used in all areas of science and technology, including the medical sciences, and they continue to pique the interest of researchers interested in discovering new dimensions for their value, which is typically related to their correspondingly small sizes. Due to its distinct relevance, silver nanoparticles have drawn particular attention among other noble metal nanoparticles. The proposed technology produces colloidal silver nanoparticles without the use of hazardous chemicals in an economical and environmentally friendly way. Plumeria rubra flower extract is vowed to help lessen and stop the reaction's agents. The produced nanoparticles are analysed by ultraviolet-visible (UV-vis) spectroscopy, Fourier transforms infrared microscopy (FTIR), x-ray diffraction (XRD) nm, and transmission electron microscope to disclose the nature of the nanoparticles (TEM). The nanoparticles have a size range of 33–53 nm. This approach produces homogenous, stable silver nanoparticles. It was discovered that the produced silver nanoparticles had improved antibacterial capabilities and displayed a zone of inhibition against particular Gram-negative and Gram-positive bacteria. Based on the findings, it can be said that plant resources can be effectively exploited to produce AgNPs, which can then be employed in a variety of industries, including biotechnology and nanotechnology.