In recent years, biological precursor-based synthesis, particularly plant-based green synthesis, has drawn prime attention in the area of nanotechnology. The goal of this work is to look into the antibacterial, antioxidant, anti-diabetic and anti-inflammatory activity of silver nanoparticles (AgNPs) made from aqueous leaf extract of Diospyroschloroxylon (Roxb).This study can be broadly separated into three parts: the first extraction from D chloroxylonleaves, second characterization of AgNPsand third analysis of AgNPspharmacological properties. UV–Vis absorption indicated the synthesis of AgNPs, and the spectrum was seen at 411 nm. The existence of functional groups in the produced AgNPs was verified by FT-IR spectral peaks that conformvarious bio-active functional groups such as alcohol, carboxylic acids, aldehyde and secondary amines including aromatic skeleton. The NPs were characterized to be in rod shape with an average particle size of 26 nm and face-centred-cubic crystal having 75.81 % metallic silver. The results of pharmacological activities of synthesized AgNPs with D chloroxylonleaf extract have significant antimicrobial, antioxidant (IC50 of74.95µg/mL), anti-inflammatory(94.71µg/mL) and anti-diabetic (124.47µg/mL) activities. As a consequence, biosynthesized Diospyroschloroxylon (Roxb.) aqueous leaf extract mediated AgNPs shown outstanding pharmacological properties and have potential application in the fields of medical and pharmaceuticals for formulation of new drugs.