The aim of the current study was to determine whether an aqueous ethanolic extract of Madhuca indica leaves had spermicidal properties. Using the in vitro immobilisation assay, different concentrations of the plant extract were used to assess the impact on rat sperm motility. The outcome revealed that when the extract was applied, all of the spermatozoa instantly became immobile at a concentration of 160 mg/ml. At a concentration of 80 mg/ml, the sperm motility was “9.81 ± 0.13, 9.70 ± 0.50, 9.52 ± 0.10, 9.38 ± 0.05, 8.89 ± 0.04 and 8.17 ± 0.01 at 0, 15, 30, 60, 120 and 180 seconds respectively”. Similar to this, reduced sperm motility was seen at concentrations of 40 mg/ml, 20 mg/ml, and 10 mg/ml. This study concludes by explaining the existence of spermicidal activity in the crude extract of Madhuca indica leaves