IJFANS International Journal of Food and Nutritional Sciences

ISSN PRINT 2319 1775 Online 2320-7876

Evaluation Of Antimicrobial Activity Of Calotropis Gigantea Extracts On Two Main Skin Infection Causing Bacteria - Escherichia Coli And Staphylococcus Aureus

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Calotropis gigantea (C. gigantea) is a wild shrub that is a medicinal plant found in abundance india china and asia region. In this study, we investigated the phytochemical composition and antibacterial properties of the haxane and ethanolic extract of C. gigantea, in addition to the antimicrobial activity of the plant and its rhizospheric actinobacteria effects against pathogenic microorganisms. Each bacterial strain was subcultured overnight at 35 oC in Mueller–Hilton Agar slants. The bacterial growth was harvested using 5 ml of sterile saline water; its absorbance was adjusted at 580 nm and diluted to attain a viable cell count of 107 CFU/ml using a spectrophotometer, and antibacterial activity shown by the disc diffusion method was used to evaluate the antimicrobial activity of the plant extract. To obtain the final concentration, the plant extract residues are dissolved in 1 ml of ethanol and haxane, sterile through Millipore filters (0.22 m), and loaded onto a sterile filter paper disc (6 mm in diameter). 10 ml of agar medium was poured into a sterile petri plate, followed by 15 ml of seeded medium previously inoculated with bacterial suspension (100 ml of medium plus 1 ml of 107 CFU) to achieve 105 CFU per ml of medium. C. procera plant extract’s antimicrobial activity was investigated using an agar well diffusion assay and minimum inhibitory concentration (MIC) against six pathogenic microbial strains. The plant extract of C. gigantea was considered significantly active against Staphylococcus aureus and Escherichia coli, with inhibition zones of 18.66 mm, 21.26 mm, and 21.93 mm, respectively, in assays.

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