This is a multicenter, randomized controlled trial focused on eight Ashtavidhi Ahar Aayatan dietary factors common in Ayurvedic concepts and practices affecting the gut microbiota. The study recruited 270 participants aged 18-65 years, distributed equally across nine groups: They are Control, Material, Cause, Association, Sign, Place, Time, Means of Tenacity, and Tenacity Instrument. Participants entered the study with prepared diets that focused on one of the eight factors, in which stool samples were collected at the beginning and the end of the intervention. The descriptive criterion of interest was the overall richness and composition of the participants’ gut microbiota. The findings of the study revealed that there was increased microbial richness in all the Ayurvedic intervention groups as compared to the control group. There were significant improvements of 0. 5-0. 8% of Bifidobacteria and Lactobacillus with p of < 0. 01 in the intervention groups and decreased F/B ratio, indicating better metabolic profiles in the intervention groups Only the Rashi, Upayoga Samstha, and Upayokta groups of Ayurvedic interventions showed significant lowering of CRP and fasting glucosaemia levels. Therefore these results imply that Ayurvedic dietary management can beneficially influence gut microbiota and support the presence of useful deformed microorganisms. A decrease in inflammatory and metabolic indicators also strengthens the hypothesis of possible therapeutic competencies of these diets. This research work forms a background for superimposing Ayurvedic dietary regimens on nutritional science to enhance the colonic status for diseases related to dysbiosis. Future studies should be done to verify these conclusions on different individuals.