IJFANS International Journal of Food and Nutritional Sciences

ISSN PRINT 2319 1775 Online 2320-7876

Effect of Different Solvents Concentrations on Total Phenolics, Flavonoids and Antioxidant Activities of Wheatgrass (Triticum aestivum)

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Renu Tripathi , Arshi , Neetu Mishra

Abstract

The purpose of this study was to investigate the effects of different solvent concentrations on the total phenolic, total flavonoid, and antioxidant activities of wheatgrass powder. The spectrophotometric method was used to analyze the total phenolic contents (TPC), total flavonoid contents (TFC), and antioxidant activities in terms of ferric-reducing antioxidant power (FRAP), DPPH radical scavenging activity, reducing capacity, and metal chelating activity. The solvents used were water, absolute ethanol, absolute methanol with aqueous ethanol, and aqueous methanol at different concentrations. The study's findings showed that, among all the solvents at different concentrations, ethanol at a 60% concentration appeared to be the most efficient solvent for extracting phenols and flavonoids from wheatgrass. It was also discovered to be the best extract, demonstrating the highest antioxidant activities. According to study findings, aqueous ethanol and methanol extracts at various concentrations displayed stronger antioxidant activity, phenolic, and flavonoid components than their absolute ones. The extraction of phenols and flavonoids from water extract was shown to be less efficient, and it also has lower antioxidant properties. Moreover, the findings of this study showed that positive and significant correlations were found between the total phenolic and flavonoid compounds and with antioxidant activities of wheatgrass extracts. In summary, it was discovered that ethanol and water mixed in a 60:40 ratio was the best solvent for extracting phenolic and flavonoid components from wheatgrass. It also proved to be the best solvent with the most antioxidant activity.

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