The velocity of ultrasound in the bio-liquids is highly useful in estimating the behaviour of the samples such as sugar level, molecular interactions. Diabetes is an inevitable in people of adults and youths due to genetic cause. Insulin plant which is used to increase the insulin level in the body and reduce the blood sugar level. The sugar solution and insulin plant extract has been mixed in various ratios and were studied. The Ultrasonic Interferometer has been used for the measurement of ultrasonic velocity(U), Ostwald Viscometer is used to determine viscosity(η); also, density(ρ), adiabatic compressibility (β) and acoustic impedance(Z) has been studied in this paper. FTIR test have been taken to determine the variations in the mixture. Using the test result, it has been concluded that there is a fall in the blood sugar level.