The goal was to analyze the effect of anaerobic training on a range of maximum power and fatigue index variables among female athletes aged 16–18, who had been training for at least two years. In order to conduct this study, twenty-four female athletes were picked at random from Punalur, Kollam district, Kerala, India. Age varied among the subjects chosen; they spanned between 16 to 18 years. With 12 participants per cluster, the sample consisted of Cluster 'I', receiving Anaerobic training, and Cluster 'II', serving as the control cluster. Cluster 'I' engaged in a training plan spanning three days, including one session every twenty-four hours. Sessions typically extended from sixty to ninety minutes. No specific training was received by members of control cluster (Cluster 'II'). Maximum power and fatigue index were measured using the running based anaerobic sprint test. Pre and post-test data collection focused on predetermined variable criteria before and following the training session. Statistical methods were applied to examine the pre and post-tests scores via dependent-'t' test and ANCOVA respectively. Examining the results, it became clear that the anaerobic training program had a substantial effect on improving maximum power and fatigue index within the experimental population. Control cluster members demonstrated neither notable advancements nor statistically meaningful differences across the key performance indicators (Maximum power and Fatigue Index).