Many diseases are caused by oxidative stress. Medicinal plants are considered as the greatest pharmaceutical stores existing on the earth as they can produce secondary phytochemicals having bioactive properties. Natural antioxidants are widely used because they are regarded as safer and causing fewer adverse. Therefore, we aimed for quantitative estimation of phytochemicals present in Aloe vera leaves and determination antioxidant activities of leaf parts of A. vera. Leaves of A. vera was subjected to successive solvent extraction by continuous hot extraction (Soxhlet) with double distilled water. The free radical scavenging activity of the aqueous (aq.) leaf extract of A. vera and standard ascorbic acid were investigated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. Results portrayed that aq. leaf extract of Aloe vera exhibited DPPH radical scavenging activity with an IC50 value of 41.59 µg/mL in comparison with the standard ascorbic acid with an IC50 value of 56.65 µg/mL. Furthermore, total phenolic quantity was found to be highest (28.12 mgGAE/g extract) in aq. leaf extract of Aloe vera when compared with total flavonoid quantities (2.44 mgQE/g extract). In conclusion, this study demonstrated the antioxidant potential of aq. leaf extract of A. vera, and this could be ascribed to the presence of phytochemicals such as phenolic compounds and flavonoids present in the aq. A. vera leaf extracts. Therefore, aq. leaf extracts of A. vera could be explored in the development of natural antioxidant drugs