This study aimed to assess the efficacy of Gorakshan product against biofilm-producing organisms. A total of 20 clinical samples were collected and processed for isolation and identification of microorganisms. Predominantly encountered organisms included S. mutans, E. coli, P. aeruginosa, S. typhi, and S. aureus from clinical samples. Biofilm-producing capability was confirmed using Congo red agar and Tube methods, with S. mutans and S. typhi identified as biofilm producers. The antimicrobial activity of Gorakshan product, specifically Snanadivilayan, Cow urine, and Panchgavya soap, demonstrated effectiveness in controlling these organisms.