Ferns are a diverse group of plant that have evolved to survive in various ecological niches. They are known to possess various biochemical constituents, which are involved in adaptation to different environmental conditions. In this study we have compared the biochemical constituent of five fern species: Actinopteris radiata, Aspleniuumpumilum, Athyrium pectinatum, A.schiemperi, Dryopteris cochleta and Hypodematiumcrenatum.The study showed that Actinopteris radiata had the highest content of total soluble sugar, starch and phenol while Aspleniuumhad the highest content of total protein and amino acid. Total free amino acid was highest in Dryopteris and Hypodematium. Athyrium had the highest soluble protein content, while Asplenium had the lowest total phenol content. These biochemical differences can be linked to ecological niche of these species and their adaptation to climate change.