Mangrove ecosystem is ideally situated at the interphase between the terrestrial and marine environment and harbors a rich and diverse group of microorganisms. Actinomycetes are the most economically and biotechnologically important prokaryotes; hold a prominent position due to their diversity and proven ability to produce novel bioactive compounds. The aim of this study was to isolate the bioactive actinomycetes strains capable of producing antimicrobial compound from the Manakudy and Rajakamangalam mangroves of Kanyakumari District, India. Here, 25 isolates of actinomycetes were isolated from the two different mangrove environment and 11 morphologically distinct isolates selected for further analysis based on antibacterial activity and extracellular enzymes production and the potent strains were identified by morphological, biochemical and genomic level identification. The isolates were preceded to preliminary screening for antibacterial activity against the bacterial pathogens by the agar overly method and cross streak method and the S4 showed highest activity against V. harveyi, S. aureus, and B. cereus in both methods. The selected 4 isolates were found to be positive for the production of industrially important enzymes such as amylase, lipase, and proteases.